[Verse 1] Prokaryote's a single loop, no nuclear wall, eukaryote's chromatin locked inside its hall. Mitochondria whisper, chloroplast still hum, old symbionts turned engines, powerhouse and photon drum. Plasmids ride like hitchhikers, extra rings of gain, viral genomes cheat the game — DNA or RNA strain, retrovirus, phage, both playing loaded dice, capsid to genome ratio — pack it once, pack it twice. [Chorus] Genoma é camada, palimpsest escrito e reescrito — layer under layer, old text never fully deleted. From the promoter to the polyA, seis quadros, sem parar Central dogma, minha bússola — DNA, RNA, proteína, vem cá [Verse 2] C-value paradox — genome size lies to your face, an onion's got more base pairs than the human race. Ploidy's the headcount, karyotype's the photo, centromere's the waistline, telomere's the coda, X and Y share borders — pseudoautosomal ground, recombining at the edges where the sex chromosomes bound. Satellite repeats coil tight around the centromere, telomeres shorten quiet every time you replicate, my dear. [Chorus] Genoma é camada, palimpsest escrito e reescrito — layer under layer, old text never fully deleted. From the promoter to the polyA, seis quadros, sem parar Central dogma, minha bússola — DNA, RNA, proteína, vem cá [Verse 3] Promoter calls the meeting, TSS strikes the gong, five prime UTR hums the intro to the song, exons hold the verses, introns get spliced away, CDS writes the message, polyA signal says okay. Codons read in triplets, one frameshift wrecks the read, ATG opens doors, a stop codon plants the seed — TAA, TAG, TGA, unless the code's rewired, mitochondrial tables swap the rules that you required. Operons in bacteria — one promoter, many genes, codon bias whispers which transcripts run the machines. [Bridge] Enhancers shout from far, silencers keep it low, insulators raise the walls so signals can't cross-flow. tRNA, rRNA, miRNA trim the fat, lncRNA and pseudogenes — echoes of what genes once were. Alu jumps, LINEs stroll, LTRs pack a punch, nucleosomes spool the thread, histones hold it close, heterochromatin's silent, euchromatin's loud and open, loops fold into TADs — compartments A and B, genome's origami, sculpture in 3D. [Verse 4] Six-frame translation — check every starting gate, ORF finder scans the codons till a stop, then weigh the freight. GFF3 gives coordinates, exon count and CDS sum, soft-masked lowercase letters, hard-masked N's struck dumb. Compare the codon usage in E. coli, fast and slow — highs pick their favorites, the lows just let the bias go. Central dogma's got exceptions — reverse transcribe, edit RNA, splice it, prion-fold it — biologia sempre nos surpreende. [Chorus] Genoma é camada, palimpsest escrito e reescrito — layer under layer, old text never fully deleted. From the promoter to the polyA, seis quadros, sem parar Central dogma, minha bússola — DNA, RNA, proteína, vem cá
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